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High Performance Liquid Chromatography/HPLC

High Performance Liquid Chromatography/HPLC

High Performance Liquid Chromatography/HPLC imadziwikanso kuti "high-pressure liquid chromatography", "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography", etc. High performance liquid chromatography ndi nthambi yofunikira chromatography.Imagwiritsa ntchito madzi monga gawo la mafoni ndipo imagwiritsa ntchito makina olowetsera othamanga kwambiri kupopera zosungunulira zokhala ndi polarities zosiyanasiyana kapena zosungunulira zosakanikirana, ma buffers ndi magawo ena oyenda ndi magawo osiyanasiyana kukhala gawo loyima.Zigawo zomwe zili mugawo la chromatographic zimapatulidwa, zimalowetsa chowunikira kuti zizindikire ndikuwunika zitsanzo.Njirayi yakhala ukadaulo wofunikira wolekanitsa ndi kusanthula m'magawo a chemistry, mankhwala, mafakitale, agronomy, kuyang'anira zinthu ndikuwunika mwalamulo.

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Makhalidwe a chromatography yamadzimadzi yogwira ntchito kwambiri:

① Kuthamanga kwambiri: Gawo la mafoni ndi madzi.Ikadutsa mugawo la chromatographic, imakumana ndi kukana kwakukulu.Kuti mudutse mwachangu pamzere wa chromatographic, madzi onyamula ayenera kukakamizidwa.

②Kuchita bwino kwambiri: kusiyanitsa kwakukulu.Gawo loyima ndi gawo loyendetsa mafoni litha kusankhidwa kuti likwaniritse bwino kwambiri kulekanitsa, komwe kumakhala kokwera kwambiri kuposa kulekanitsa kwa nsanja za distillation za mafakitale ndi chromatography ya gasi.

③Kukhudzika kwakukulu: Chowunikira cha UV chimatha kufikira 0.01ng.

④Kugwiritsa ntchito kosiyanasiyana: Kupitilira 70% yazinthu zachilengedwe zitha kuwunikidwa ndi chromatography yamadzimadzi kwambiri.

⑤ Kuthamanga kwachangu komanso kuthamanga kwamadzi onyamula: mwachangu kwambiri kuposa chromatography yamadzimadzi

Kuphatikiza apo, ma chromatography amadzimadzi apamwamba kwambiri ndi ma chromatography angagwiritsidwe ntchito mobwerezabwereza, zitsanzo siziwonongeka, ndipo ndizosavuta kuchira.Komabe, amakhalanso ndi zovuta zake.Poyerekeza ndi chromatography ya gasi, aliyense ali ndi zabwino zake ndipo amathandizirana.
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Nthawi yotumiza: Nov-23-2023